Introduction

Here, we describe integrative modeling using the PMI interface and then demonstrate its use by modeling the complex of actin and tropomodulin-gelsolin chimera using SAXS, EM, crosslinking, crystal structures of the individual domains and physical principles:

• Description of the design of the PMI software
• Modeling of the actin-tropomodulin-gelsolin complex
• Analysis of the actin complex models and deposition

First, on to the design of the PMI software.