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Integrative structures of the A3G-CRL5-Vif-CBFb complex

Integrative structure determination of the A3G-CRL5-Vif-CBFβ complex PubMed logo PDB-Dev

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Integrative structure determination of the A3G-VifHIV-1-CRL5-CBFβ complex.


Integrative structure determination of the A3G-CRL5-Vif-CBFβ complex based on the 132 DSSO cross-links, atomic structures of the components, and previously published mutagenesis data. Preliminary integrative modeling represented the CRL5-Vif-CBFβ complex by a single rigid body defined by atomic structure of the Vif-CBFβ-Cul5N-term-EloB-EloC subcomplex (PDB access code: 4N9F) and comparative models of the full length Cul5 and Rbx2 (rigid representation). Following, to characterize the flexibility of the complex, we performed integrative structure modeling of the A3G-CRL5-Vif-CBFβ complex with a modified representation that allowed for relaxation in the configuration of the VCBC subcomplex subunits and for alternative conformations of Cul5 and A3G subunits (flexible representation). The structure was cross-validated based on the data used for modeling and previously published structural, biochemical, and functional data not used for modeling.

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Author (s): Ignacia Echeverria

License: CC BY-SA 4.0 This work is licensed under the Creative Commons Attribution-ShareAlike 4.0 International License.

Publications: Characterization of a A3G-VifHIV-1-CRL5-CBFβ structure using a cross-linking mass spectrometry pipeline for integrative modeling of host-pathogen complexes. Robyn M. Kaake, Ignacia Echeverria, Seung Joong Kim, John Von Dollen, Nicholas M. Chesarino, Yuqing Feng, Clinton Yu, Hai Ta, Linda Chelico, Lan Huang, John Gross, Andrej Sali, Nevan J. Krogan, DOI: