PMI RNAPII Modeling Tutorial

Tutorial that demonstrates the modeling of RNA Polymerase II using Electron Microscopy density and chemical crosslinks

Verified to work with the latest stable IMP release (2.22.0). The files are also available at GitHub.

Additional software needed to use these files: IMP scikit-learn matplotlib install instructions

Anaconda logo To install the software needed to reproduce this system with the Anaconda Python command line tool (conda), run the following commands:

conda config --add channels salilab
conda install imp scikit-learn matplotlib

UCSF logo To set up the environment on the UCSF Wynton cluster to run this system, run:

module load Sali
module load imp python3/scikit python3/matplotlib

IMP is a tool to generate structural models based on multiple sources of experimental data. A detailed explanation of IMP and integrative modeling is given here

This tutorial explores scripts that:

Show the basic capabilities of IMP
Sample models of RNA Polymerase II using Electron Microscopy density and chemical crosslinks
Visualize these models and analyze them for accuracy and precision

Last known good IMP version:

Running the Tutorial

See the IMP website for the tutorial text.