|
Hello,
I'm trying to set up PMI GaussianEMRestraints with proteins consisting of flexible beads and rigid bodies. I followed the approach of the RNA Pol tutorial, so I used IMP.pmi.tools.get_densities and pass it on to IMP.pmi.restraints.em.GaussianEMRestraint.
The GMM was created from a map via create_gmm.py.
However, it seems that only the flexible beads are being restrained and the rigid bodies are not fitted to the GMM. Any idea why this is happening? I also have a simpler test case prepared if necessary.
Best, Swantje
--
Swantje Lenz
M. Sc. Biotechnology Fachgebiet Bioanalytik (TIB 4/4-3), Institut für Biotechnologie, Technische Universität Berlin
Technische Universität Berlin | Gustav-Meyer-Allee 25 | Gebäude 17a | 13355 Berlin Tel +49 30 314-72906 | web: http://www.bioanalytik.tu-berlin.de/ |