On 10/20/16 7:01 PM, Julian wrote:
I'm trying to figure out how one would use the IDEAL_HELIX representation for purposes of EMRestraint.
...
When I was modeling Rpb4 and Rpb7 as BEADS, everything seemed to be working. I then tried modeling a stretch of Rpb4 as IDEAL_HELIX, but I'm not sure the density of that helix is recognized and the representation/density doesn't look they way I would expect in Chimera.
Can you be a bit more specific about the problem? An ideal helix is basically the same as a set of beads, only that the initial positions of the 1-residue beads are set along a spiral. (The helix will fall apart during modeling though unless you also make it a rigid body.)
Ben -- https://salilab.org/~ben/ "It is a capital mistake to theorize before one has data." - Sir Arthur Conan Doyle