IMP
2.3.1
The Integrative Modeling Platform
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Fit atomic structures into EM density maps.
MultiFit is a protocol for simultaneously fitting atomic structures of components into the cryo-electron microscopy (cryo-EM) density map of their assembly. It can also incorporate connectivity information from proteomics experiments (such as cross links), and can work with density maps at resolutions as low as 25A.
See also cnmultifit for a similar protocol operating on symmetric complexes.
The component positions and orientations are optimized with respect to a scoring function that includes the quality-of-fit of components in the map, the protrusion of components from the map envelope, and the shape complementarity between pairs of components. The scoring function is optimized by an exact inference optimizer DOMINO that efficiently finds the global minimum in a discrete sampling space. The protocol employs the IMP::multifit module.
A webserver is also available.
Examples:
Build an anchor graph from a PDB file.
Author(s): Keren Lasker
Maintainer: benmwebb
License: LGPL This library is free software; you can redistribute it and/or modify it under the terms of the GNU Lesser General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version.
Publications: