A protocol for generating atomic structures of complexes with cyclic symmetry (such as rings), using only the atomic structure of a single subunit and a cryo-electron microscopy density map of the complex. This employs the IMP::cnmultifit module.

See also multifit for a similar protocol operating on non-symmetric complexes.

A webserver is also available.

Examples:

Modeling of GroEL

Info

Author(s): Keren Lasker

Maintainer: benmwebb

License: LGPL This library is free software; you can redistribute it and/or modify it under the terms of the GNU Lesser General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version.

Publications:

Keren Lasker, Maya Topf, Andrej Sali, “Determining macromolecular assembly structures by molecular docking and fitting into an electron density map”, Journal of Molecular Biology, 2009.
Keren Lasker, Daniel Russel, Jeremy Phillips, Haim Wolfson, Andrej Sali, “Determining architectures of macromolecular assemblies by aligning interaction networks to electon microscopy density maps”, submitted