IMP
2.2.0
The Integrative Modeling Platform
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EMageFit is an application to build models of macromolecular assemblies using restraints from EM images (class-averages).
Apart from EM images, the method relies on other types of restraints:
EMageFit samples for good conformations of the macromolecular assembly using multiple molecular docking (optional, but it can be very useful), Simulated Annealing Monte Carlo optimization, and sampling with the discrete optimizer DOMINO. It is also straightforward to incorporate other restraints to the method.
If available, EMageFit will use the docking program HEXDOCK to do docking between subunits that are related by cross-links. But it is worth mentioning that EMageFit can also work with any other docking program, or simply using the cross-linking restraints and no docking at all.
More detail on the functioning of EMageFit can be found on the protocol page, and many of the scripts and utilities are documented on the scripts page.
Examples:
Author(s): Javier Velazquez-Muriel
Maintainer: benmwebb
License: LGPL This library is free software; you can redistribute it and/or modify it under the terms of the GNU Lesser General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version.
Publications: